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Article in Journal ()

Oscillating Magnet Array-Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies

Citation
Subramanian M, Tyler A, Luther EM, Di Daniel E, Lim J & Dobson J (2017) Oscillating Magnet Array-Based Nanomagnetic Gene Transfection: A Valuable Tool for Molecular Neurobiology Studies, Nanomaterials, 7 (2), Art. No.: 28.

Abstract
To develop treatments for neurodegenerative disorders, it is critical to understand the biology and function of neurons in both normal and diseased states. Molecular studies of neurons involve the delivery of small biomolecules into cultured neurons via transfection to study genetic variants. However, as cultured primary neurons are sensitive to temperature change, stress, and shifts in pH, these factors make biomolecule delivery difficult, particularly non-viral delivery. Herein we used oscillating nanomagnetic gene transfection to successfully transfect SH-SY5Y cells as well as primary hippocampal and cortical neurons on different days in vitro. This novel technique has been used to effectively deliver genetic material into various cell types, resulting in high transfection efficiency and viability. From these observations and other related studies, we suggest that oscillating nanomagnetic gene transfection is an effective method for gene delivery into hard-to-transfect neuronal cell types.

Keywords
magnetic nanoparticles; transfection; SH-SY5Y; hippocampal neurons; cortical neurons; non-viral gene delivery; nanomagnetic gene transfection; biomagnetics; gradient magnetic field; permanent magnets

StatusPublished
AuthorsSubramanian Mahendran, Tyler Aimee-Jayne, Luther Eva Maria, Di Daniel Elena, Lim Jenson, Dobson John
Publication date29/01/2017
Publication date online29/01/2017
Date accepted by journal23/01/2017
URLhttp://www.mdpi.com/2079-4991/7/2/28
PublisherMDPI
ISSN 2079-4991
LanguageEnglish

Journal
Nanomaterials: Volume 7, Issue 2

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