Research output

Article in Journal ()

Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays

Citation
Cruz VP, Vera M, Pardo BG, Taggart J, Martinez P, Oliveira C & Foresti F (2017) Identification and validation of single nucleotide polymorphisms as tools to detect hybridization and population structure in freshwater stingrays, Molecular Ecology Resources, 17 (3), pp. 550-556.

Abstract
Single nucleotide polymorphism (SNP) markers were identified and validated for two stingrays species, Potamotrygon motoro and Potamotrygon falkneri, using double digest restriction-site associated DNA (ddRAD) reads using 454-Roche technology. A total of 226774 reads (65.5Mb) were obtained (mean read length 289±183bp) detecting a total of 5399 contigs (mean contig length: 396±91bp). Mining this data set, a panel of 143 in silico SNPs was selected. Eighty-two of these SNPs were successfully validated and 61 were polymorphic: 14 in P.falkneri, 21 in P.motoro, 3 in both species and 26 fixed for alternative variants in both species, thus being useful for population analyses and hybrid detection.

Keywords
conservation tools; genetic fish; hybridization; NGS techniques; Potamotrygon ; SNPs

StatusPublished
AuthorsCruz Vanessa P, Vera Manuel, Pardo Belen G, Taggart John, Martinez Paulino, Oliveira Claudio, Foresti Fausto
Publication date05/2017
Publication date online28/07/2016
Date accepted by journal07/01/2016
PublisherWiley-Blackwell
ISSN 1755-098X
LanguageEnglish

Journal
Molecular Ecology Resources: Volume 17, Issue 3 (2016)

© University of Stirling FK9 4LA Scotland UK • Telephone +44 1786 473171 • Scottish Charity No SC011159
My Portal