Research output

Article in Journal ()

Exercise-conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells

Alternative title Exercise-Conditioned Plasma and DNMT3B Concentration in PBMCs

Citation
Horsburgh S, Todryk S, Toms C, Moran CN & Ansley L (2015) Exercise-conditioned plasma attenuates nuclear concentrations of DNA methyltransferase 3B in human peripheral blood mononuclear cells, Physiological Reports, 3 (12), Art. No.: e12621.

Abstract
DNA methylation is modifiable by acute and chronic exercise. DNA methyltransferases (DNMT) catalyze this process; however, there is a lack of literature concerning the specific mechanisms by which exercise‐induced modifications occur. Interleukin 6 (IL‐6) stimulation of various cell lines has been shown to augment DNMT expression and nuclear translocation, which suggests a possible pathway by which exercise is able to elicit changes in epigenetic enzymes. The present study sought to elucidate the response of thede novomethyltransferases DNMT3A and DNMT3B to circulatory factors found in plasma isolated from whole blood before and after 120‐min of treadmill running at an intensity of 60% of individual velocity at(v) interspersed with 30‐sec sprints at 90% of vevery 10‐min. Peripheral blood mononuclear cells (PBMCs) isolated from a resting participant were incubated with plasma isolated from exercising participants (n=10) or recombinant IL‐6 (rIL‐6), followed by nuclear protein extraction and quantification of DNMT3A and DNMT3B concentrations. Nuclear concentrations of DNMT3B significantly decreased following the experimental protocol (P=0.03), with no change observed in DNMT3A (P=0.514).Various concentrations of rIL‐6 caused an elevation in both DNMT3A and DNMT3B nuclear concentration compared with the blank control. The conflicting results between exercising and rIL‐6 conditions suggests that IL‐6 does regulate DNMT nuclear transport, however, other plasma mediators may also exert significant influence on the nuclear concentrations of these enzymes.

Keywords
DNA methylation; epigenetics; inflammation

StatusPublished
AuthorsHorsburgh Steven, Todryk Stephen, Toms Christopher, Moran Colin Neil, Ansley Les
Publication date10/12/2015
Publication date online10/12/2015
Date accepted by journal19/10/2015
PublisherWiley for the American Physiological Society and The Physiological Society
ISSN 2051-817X
LanguageEnglish

Journal
Physiological Reports: Volume 3, Issue 12

© University of Stirling FK9 4LA Scotland UK • Telephone +44 1786 473171 • Scottish Charity No SC011159
My Portal