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Examination of the early infection stages of koi herpesvirus (KHV) in experimentally infected carp, Cyprinus carpio L. using in situ hybridisation

Alternative title Early stages of KHV using ISH

Citation
Monaghan S, Thompson K, Adams A, Kempter J & Bergmann S (2015) Examination of the early infection stages of koi herpesvirus (KHV) in experimentally infected carp, Cyprinus carpio L. using in situ hybridisation, Journal of Fish Diseases, 38 (5), pp. 477-489.

Abstract
Koi herpesvirus (KHV) causes a highly infectious disease afflicting common carp and koi,Cyprinus carpioL. Various molecular and antibody-based detection methods have been used to elucidate the rapid attachment and dissemination of the virus throughout carp tissues, facilitating ongoing development of effective diagnostic approaches.In situhybridization (ISH) was used here to determine the target tissues of KHV during very early infection, after infecting carp with a highly virulent KHV isolate. Analysis of paraffin-embedded tissues (i.e. gills, skin, spleen, kidney, gut, liver and brain) during the first 8h and following 10days post-infection (hpi; dpi) revealed positive signals in skin mucus, gills and gut sections after only 1hpi. Respiratory epithelial cells were positive as early as 2hpi. Viral DNA was also detected within blood vessels of various tissues early in the infection. Notable increases in signal abundance were observed in the gills and kidney between 5 and 10 dpi, and viral DNA was detected in all tissues except brain. This study suggests that the gills and gut play an important role in the early pathogenesis of thisAlloherpesvirus,in addition to skin, and demonstrates ISH as a useful diagnostic tool for confirmation of acutely infected carp.

Keywords
cyprinid herpesvirus 3;early infection;in situ hybridization;koi herpesvirus;pathogenesis

StatusPublished
AuthorsMonaghan Sean, Thompson Kimberly, Adams Alexandra, Kempter Jolanta, Bergmann Sven
Publication date05/2015
Publication date online13/06/2014
Date accepted by journal20/03/2014
PublisherWiley-Blackwell
ISSN 0140-7775
LanguageEnglish

Journal
Journal of Fish Diseases: Volume 38, Issue 5

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