Research output

Chapter (in Edited Book) ()

Methods in Capillary Electrophoresis Coupled to Mass Spectrometry for the Identification of Clinical Proteomic/Peptidomic Biomarkers in Biofluids

Citation
Stalmach A, Husi H, Mosbahi K, Albalat A, Mullen W & Mischak H (2015) Methods in Capillary Electrophoresis Coupled to Mass Spectrometry for the Identification of Clinical Proteomic/Peptidomic Biomarkers in Biofluids. In: Vlahou A, Makridakis M (ed.). Clinical Proteomics: Methods and Protocols. Methods in Molecular Biology, 1243, New York: Springer, pp. 187-205.

Abstract
Proteomic biomarkers hold the promise of enabling assessment of patients according to a pathological condition aiming at improvements in diagnosis, prognosis, in general clinical patient management. Capillary electrophoresis coupled to an electrospray ionization time-of-flight mass spectrometer (CE-MS) allows the detection of thousands of small proteins and peptides in various biofluids, in a single, reproducible and time-limited step, enabling the simultaneous comparison of multiple individual proteins and peptides in biomarker discovery, but also in clinical applications. The reliability of the CE-MS platform, together with the use of a validated approach for data processing and mining is, to date, the most advanced technique for biomarker discovery of clinical significance. In this chapter, we report on the materials, methods and protocols used for CE-MS-based clinical proteomics allowing the reproducible profiling of biofluids.

Keywords
Capillary electrophoresis; Mass spectrometry; Peptide; Biomarker; Bodyfluid

StatusPublished
EditorVlahou A, Makridakis M
AuthorsStalmach Angelique, Husi Holger, Mosbahi Khedidja, Albalat Amaya, Mullen William, Mischak Harald
Title of seriesMethods in Molecular Biology
Number in series1243
Publication date2015
URLhttp://link.springer.com/…1-4939-1872-0_11
PublisherSpringer
Place of publicationNew York
ISSN of series 1064-9745
ISBN 978-1-4939-1871-3
LanguageEnglish
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