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Autophagy-inducing peptides from mammalian VSV and fish VHSV rhabdoviral G glycoproteins (G) as models for the development of new therapeutic molecules

Citation
Garcia-Valtanen P, Ortega-Villaizan MdM, Martinez-Lopez A, Medina-Gali R, Perez L, MacKenzie S, Figueras A, Coll JM & Estepa A (2014) Autophagy-inducing peptides from mammalian VSV and fish VHSV rhabdoviral G glycoproteins (G) as models for the development of new therapeutic molecules, Autophagy, 10 (9), pp. 1666-1680.

Abstract
It has not been elucidated whether or not autophagy is induced by rhabdoviral G glycoproteins (G) in vertebrate organisms for which rhabdovirus infection is lethal. Our work provides the first evidence that both mammalian (vesicular stomatitis virus, VSV) and fish (viral hemorrhagic septicemia virus, VHSV, and spring viremia carp virus, SVCV) rhabdoviral Gs induce an autophagic antiviral program in vertebrate cell lines. The transcriptomic profiles obtained from zebrafish genetically immunized with either Gsvcv or Gvhsv suggest that autophagy is induced shortly after immunization and therefore, it may be an important component of the strong antiviral immune responses elicited by these viral proteins. Pepscan mapping of autophagy-inducing linear determinants of Gvhsv and Gvsv showed that peptides located in their fusion domains induce autophagy. Altogether these results suggest that strategies aimed at modulating autophagy could be used for the prevention and treatment of rhabdoviral infections such as rabies, which causes thousands of human deaths every year.

Keywords
antiviral; autophagy; immune response; LC3; microarrays; pepscan; rhabdovirus; SVCV; VHSV; viral glycoprotein; VSV; zebrafish

StatusPublished
AuthorsGarcia-Valtanen Pablo, Ortega-Villaizan Maria del Mar, Martinez-Lopez Alicia, Medina-Gali Regla, Perez Luis, MacKenzie Simon, Figueras Antonio, Coll Julio M, Estepa Amparo
Publication date09/2014
Date accepted by journal11/06/2014
PublisherTaylor and Francis
ISSN 1554-8627
LanguageEnglish

Journal
Autophagy: Volume 10, Issue 9 (2014)

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