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In vivo metabolism of unsaturated fatty acids in Octopus vulgaris hatchlings determined by incubation with 14C-labelled fatty acids added directly to seawater as protein complexes

Citation
Reis DB, Acosta NG, Almansa E, Navarro JC, Tocher DR, Monroig O, Andrade JP, Sykes AV & Rodriguez C (2014) In vivo metabolism of unsaturated fatty acids in Octopus vulgaris hatchlings determined by incubation with 14C-labelled fatty acids added directly to seawater as protein complexes, Aquaculture, 431, pp. 28-33.

Abstract
The high mortalities observed during Octopus vulgaris paralarvae culture have been associated with a nutritional imbalance, with long-chain polyunsaturated fatty acids (LC-PUFA) appearing to have a critical role. In order to determine the in vivo capability of O. vulgaris hatchlings to incorporate and metabolise unsaturated fatty acids (FA), hatchlings were incubated in flat-bottom 6-well tissue culture plates at a density of 90 hatchlings/well in 10 mL of seawater (36‰). Incubations were performed with gentle stirring at 21 °C for 6 h with 0.2 μCi (0.3 μM) of [1−14C]-labelled FA including 18:1n−9, 18:2n−6, 18:3n−3, 20:4n−6 (ARA), 20:5n−3 (EPA) or 22:6n−3 (DHA), which were added directly to the seawater as their potassium salts bound to bovine serum al- bumin (BSA). A control treatment without [1−14C]FA was also assessed. O. vulgaris hatchlings not only possessed the ability to incorporate FA bound to BSA, but also to esterify them into phospholipid, with marked specificity. [1−14C]DHA and [1−14C]C18 FA substrates were mainly esterified into phosphatidylcholine, while [1−14C]ARA and [1−14C]EPA were esterified into phosphatidylethanolamine. The majority of radioactivity from [1−14C]FA incorporated into hatchling total lipid was recovered as unmodified FA with elongation being the only metabo- lism detected. Of the FA investigated, [1−14C]ARA was the most efficiently incorporated into hatchling lipids, but it was also the least modified FA. The fact that no desaturation activity was recorded towards the FA tested in this experiment may indicate that the nutritional requirements of O. vulgaris hatchlings in terms of FA are highly specific and LC-PUFA must be considered essential dietary nutrients.

Keywords
Common octopus; Lipid metabolism; Octopus vulgaris; hatchlings; Radiolabeled substrates; Unsaturated fatty acids

StatusPublished
AuthorsReis D B, Acosta Nieves Guadalupe, Almansa Eduardo, Navarro Juan Carlos, Tocher Douglas R, Monroig Oscar, Andrade Jose Pedro, Sykes Antonio V, Rodriguez Covadonga
Publication date20/07/2014
Publication date online09/05/2014
Date accepted by journal09/05/2014
PublisherElsevier
ISSN 0044-8486
LanguageEnglish

Journal
Aquaculture: Volume 431 (2014)

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