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Effects of nauplial density, product concentration and product dosage on the survival of the nauplii and EFA incorporation during Artemia enrichment with liposomes

Citation
Monroig O, Navarro JC, Amat F, Gonzalez P & Hontoria F (2006) Effects of nauplial density, product concentration and product dosage on the survival of the nauplii and EFA incorporation during Artemia enrichment with liposomes, Aquaculture, 261 (2), pp. 659-669.

Abstract
In order to determine an optimal enrichment protocol with krill phospholipid liposomes, three different experiments were carried out to evaluate the effects of nauplial density, the concentration of liposome, and the number of doses delivered to the nauplii on the survival and the essential fatty acid (EFA) bioencapsulation in Artemia nauplii. No survival differences were found between the different liposome treatments and the control nauplii enriched with a commercial emulsion. This result confirms that previously described mortalities during Artemia enrichment with liposomes can be minimized and controlled. On the other hand, the EFA incorporation obtained from the three experiments indicates that maximal bioencapsulation is achieved when incubations are carried out under nauplial densities of 300 nauplii ml- 1, where liposomes are dispensed in a single dose at the beginning of the incubation yielding a concentration of 0.5 g l- 1. These findings complete those obtained in two preliminary studies, and propose a well defined enrichment protocol with krill liposomes as an alternative complementary method to improve the nutritional value of the Artemia nauplii used as food for marine fish larvae.

Keywords
enrichment protocol optimization; nauplial density; liposome concentration; liposome dosage; nauplial survival

StatusPublished
AuthorsMonroig Oscar, Navarro Juan Carlos, Amat Francisco, Gonzalez Pedro, Hontoria Francisco
Publication date24/11/2006
PublisherElsevier
ISSN 0044-8486
LanguageEnglish

Journal
Aquaculture: Volume 261, Issue 2 (NOV 24 2006)

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