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Sensitivity of seven PCRs for early detection of koi herpesvirus in experimentally infected carp, Cyprinus carpio L., by lethal and non-lethal sampling methods

Alternative title Early KHV detection by 7 PCRs

Citation
Monaghan S, Thompson K, Adams A & Bergmann SM (2015) Sensitivity of seven PCRs for early detection of koi herpesvirus in experimentally infected carp, Cyprinus carpio L., by lethal and non-lethal sampling methods, Journal of Fish Diseases, 38 (3), pp. 303-319.

Abstract
Koi herpesvirus (KHV) causes an economically important, highly infectious disease in common carp and koi, rence of mass mortalities worldwide, highly spe- cific and sensitive molecular diagnostic methods have been developed for KHV detection. The sen- sitivity and reliability of these assays have essen- tially focused at the detection of low viral DNA copy numbers during latent or persistent infec- tions. However, the efficacy of these assays has not been investigated with regard to low-level viraemia during acute infection stages. This study was conducted to compare the sensitivity of seven different polymerase chain reaction (PCR) assays to detect KHV during the first hours and days post-infection (hpi; dpi), using lethal and non- lethal sampling methods. The results highlight the limitations of the assays for detecting virus during the first 4 dpi despite rapid mortality in experi- mentally infected carp. False-negative results were associated with time post-infection and the tissue sampled. Non-lethal sampling appears effective for KHV screening, with efficient detection in mucus samples obtained from external swabs during this early infection period ( from gills and kidney were negative using the same PCR assays. Non-lethal sampling may improve the reliability of KHV detection in sub- clinical, acutely infected carp.Cyprinus carpio L. Since the occur-<5 dpi), while biopsies

Keywords
cyprinid herpesvirus 3; early detection; Koi herpesvirus; non-lethal sampling; polymerase chain reaction

StatusPublished
AuthorsMonaghan Sean, Thompson Kimberly, Adams Alexandra, Bergmann Sven M
Publication date03/2015
Date accepted by journal13/01/2014
PublisherWiley-Blackwell
ISSN 0140-7775
LanguageEnglish

Journal
Journal of Fish Diseases: Volume 38, Issue 3

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