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Article in Journal ()

Patterns of transgene inheritance in rainbow trout (Oncorhynchus mykiss)

Citation
Penman D, Iyengar A, Beeching AJ, Rahman MA, Sulaiman Z & Maclean N (1991) Patterns of transgene inheritance in rainbow trout (Oncorhynchus mykiss), Molecular Reproduction and Development, 30 (3), pp. 201-206.

Abstract
There have been very few studies of the inheritance of introduced genes (transgenes) in fish. We have followed the inheritance of the mammalian fusion gene MTrGH from founder generation transgenics (originating from eggs microinjected with the MTrGH DNA) to offspring in crosses with control fish. Initial screening of the founder generation transgenics was by analysing DNA from blood samples. Only three out of six fish which carried the novel gene in blood DNA transmitted it to their offspring, despite the presence of the gene in DNA extracted from the sperm of all four male fish in this group. The frequency of transgenics in the progeny groups from the three fish which transmitted the gene varied widely: in one of these groups more than one type of MTrGH restriction pattern was found. These results suggest widespread mosaicism in founder generation transgenics.

Keywords
Transgene inheritance; Rainbow trout; Founder generation transgenics; DNA; MTrGH

StatusPublished
AuthorsPenman David, Iyengar Arati, Beeching A J, Rahman M Azizur, Sulaiman Zorah, Maclean Norman
Publication date11/1991
PublisherWiley-Blackwell
ISSN 1040-452X
LanguageEnglish

Journal
Molecular Reproduction and Development: Volume 30, Issue 3 (NOV 1991)

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