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Physical mapping of the brain and ovarian aromatase genes in the Nile Tilapia, Oreochromis niloticus, by fluorescence in situ hybridization

Citation
Harvey SC, Kwon JY & Penman D (2003) Physical mapping of the brain and ovarian aromatase genes in the Nile Tilapia, Oreochromis niloticus, by fluorescence in situ hybridization, Animal Genetics, 34 (1), pp. 62-64.

Abstract
Cytochrome P450-aromatase enzyme (CYP19), which catalyses the conversion of androgens to oestrogens, is critical in ovarian differentiation and hence in the sex differentiation pathways of non-mammalian vertebrates. As in other fish species, distinct ovarian and brain aromatase genes have been identified in the Nile Tilapia, Oreochromis niloticus. Here we demonstrate by in situ hybridization that the two aromatase genes of this species are present on different chromosomes and that neither are located on the sex chromosomes. Hence, the aromatase genes are not the primary sex determination genes in O. niloticus.

Keywords
aromatase; bacterial artificial chromosome; fluorescence in situ hybridization; Oreochromis niloticus; P450; Tilapia

StatusPublished
AuthorsHarvey Simon C, Kwon Joon Yeong, Penman David
Publication date02/2003
PublisherWiley-Blackwell
ISSN 0268-9146
LanguageEnglish

Journal
Animal Genetics: Volume 34, Issue 1 (2003)

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